Composite

Part:BBa_K4425013:Design

Designed by: Junfei Xia   Group: iGEM22_SUSTech_Shenzhen   (2022-09-30)


pET28a-BGL


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 190
    Illegal EcoRI site found at 196
    Illegal XbaI site found at 47
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 190
    Illegal EcoRI site found at 196
    Illegal NheI site found at 151
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 190
    Illegal EcoRI site found at 196
    Illegal BamHI site found at 184
    Illegal XhoI site found at 1618
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 190
    Illegal EcoRI site found at 196
    Illegal XbaI site found at 47
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 190
    Illegal EcoRI site found at 196
    Illegal XbaI site found at 47
    Illegal AgeI site found at 529
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

a


Source

a

References